Figure 5
LPS purified from Escherichia coli (EL) caused anxiety and colitis in mice. Experimental schedule (a). Anxiety-like behaviors were measured on the 10th day after the initial treatment with LPS in the EPM (b) MB (d) and LDT (d) tasks. LPS (10 μg/kg) was intraperitoneally treated for 5 days. NF-κB activation and BDNF and claudin-5 expression were measured in the hippocampus (e). Brain TNF-α (f) IL-1β (g) and IL-6 (h) levels were analyzed by ELISA kits. Blool corticosterone (i) IL-6 (f) TNF-α (j) IL-1β (k) IL-6 (l) and LPS (m) levels were by ELISA kits. Colitis markers body weight gain (n) macroscopic score (o) colon shortening (p) and MPO activity (q) IL-6 (r) TNF-α (s) IL-1β (t) and IL-10 (u) expression were measured in the colon. NC and EL in figures indicate groups treated with vehicle alone (1% dextrose) and E. coli LPS, respectively. All data except immunoblotting data (n = 4) were expressed as mean ± SD (n = 8). #p < 0.05 vs. NC group.
