Table 1 Components described on CA by immunohistochemical studies.
From: Exploring the elusive composition of corpora amylacea of human brain
Antibody | Epitope | CA staining | Ref. |
---|---|---|---|
Directed against Tau protein | |||
Tau-2 | phosphatase-independent epitope of tau | + | |
Tau 1, clone PC1C6 | non-phosphorylated epitope of tau | − | |
PHF-1 | Tau phosphorylated on ser396 and ser404 | + | |
TauC3 | caspase cleaved tau truncated at asp421 | + | |
AT8 | tau phosphorylated on ser202 and thr205 | − | |
HT7 | full-lenght tau | − | |
Tau46 | tau C-terminal specific | + | |
AT8 | tau phosphorylated at ser202 and thr205 | − | |
Tau-2 | human tau | + | |
PHF, clone AT100 | tau phosphorylated on thr212 and ser214 | − | |
Tau pS422 | tau phosphorylated on ser422 | − | |
Tau-5 | tau 210–241 aminoacids | − | |
5E2 | tau | − | |
Directed against Amyloid-β (Aβ) or amyloid-β protein precursor (APP) | |||
4G8 | Aβ 17-24 aminoacids | − | |
anti-Aβ 1-42 | human Aβ 1-42 | − | |
anti-Aβ | Aβ 1-40/42 | + | |
P2 | extracytoplasmic APP site | + | |
APP-A4, clone 22C11 | N-terminal domain of APP | + | |
anti-Aβ1–42, 12F4 | Aβ 1-42 aminoacids | − | |
anti-Aβ1–16, 6E10 | Aβ 1-16 aminoacids | − | |
Directed against nestin | |||
anti-nestin | nestin | − | |
anti-nestin | nestin | + | |
Directed against NeuN | |||
anti-NeuN | NeuN protein | +(some) | |
anti-NeuN | NeuN protein | + | |
anti-NeuN | NeuN protein | + | |
Directed against synuclein (syn) | |||
α/β/γ-syn, FL-140 | human synuclein | + | |
anti-α-syn | α-synuclein protein | + | |
anti-α-syn | α-synuclein protein | +(some) | |
Directed against parkin | |||
anti-parkin | human parkin | + | |
Directed against reelin | |||
G10 | reelin N-terminus | + | |
clone 142 | reelin N-terminus | + | |
R12/14 | reelin C-terminus | + | |
Directed against microtubule-associated protein 2 (MAP2) | |||
anti-MAP2 | MAP2 | + | |
anti-MAP2 | MAP2 | + | |
Directed against neurofilaments | |||
2F11 | Neurofilament protein | +(some) | |
anti-NF70 | Neurofilament protein | −(one+) | |
Directed against S100 | |||
S100 antisera | S100 proteins (different types, not S100B) | + | |
S100B antisera | S100B protein | − | |
anti-S100 | glial S100 protein | + | |
anti-S100 | glial S100 protein | + | |
anti-S100 | glial S100 protein | + | |
Directed against glial fibrillary acidic protein (GFAP) | |||
anti- GFAP | GFAP | +(some) | |
anti-GFAP | GFAP | + | |
anti-GFAP | GFAP | outlining | |
anti-GFAP | GFAP | outlining | |
anti-GFAP | GFAP | outlining | |
anti-GFAP | GFAP | outlining | |
Directed against ubiquitin | |||
anti-ubiquitin | ubiquitin | + | |
anti-ubiquitin | ubiquitin | + | |
anti-ubiquitin | human ubiquitin | + | |
anti-ubiquitin | human ubiquitin | + | |
anti-ubiquitin | ubiquitin | + | |
Directed against heat-shock proteins (HSP) | |||
anti-HSP70 | HSP70 | + | |
anti-HSP27 | HSP27 | −(some outlining) | |
anti-HSP70-72 | HSP70-72 | −(some outlining) | |
anti-HSP90 | HSP90 | −(some outlining) | |
anti-HSP28 | HSP28 | + | |
anti-HSP70 | HSP70 | + | |
anti-HSP72 | HSP72 | outlining/+ | |
anti-HSP60 | HSP60 383-447 | + | |
Directed against aquaporin 4 (AQP4) | |||
anti-aquaporin 4 | AQP4 | + | |
anti-aquaporin 4 | AQP4 | outlining | |
Directed against advanced glycation end-products (AGE) | |||
6D12 | CML (N-(carboxymethyl)lysine) | + | |
anti-pentosidine | pentosidine | + | |
Directed against proteoglycans | |||
anti-proteoglycan | keratan sulfate proteoglycan | + | |
anti-glycoconjugage | mannose rich glycoconjugate | + | |
Directed against mitochondrial (mit.) epitopes | |||
anti-Bcl-2 | mit. membrane associated protein Bcl-2 | + | |
Directed against transcriptional factors | |||
anti-c-Jun/AP1 | activator prot. 1 transcriptional factor c-Jun | + | |
Directed against transglutaminases (TGs) or products generated by TG | |||
anti-TG2 | guinea pig TG2 | − | |
anti-TG1 | human TG1 | + | |
81D4 | H-Glu(H-Lys-OH)-OH | + | |
Directed against cytoskeletal proteins | |||
anti-β-tubulin III | β-tubulin III | + | |
anti-β-actin | β-actin | + | |
Directed against thrombospondin | |||
A6.1 | thrombospondin1 | + | |
SPM321 | thrombospondin1 | + | |
Directed against ADAMTS3 | |||
anti-ADAMTS13 | ADAMTS13 | + | |
Directed against hypoxia-inducible factor 1-alpha (HIF-1α) | |||
anti-HIF-1α | HIF-1α | + | |
Directed against endothelin B receptor | |||
anti-ETBR | endothelin B receptor | + | |
Directed against fungal proteins | |||
antifungal antibodies | C. glabrata, C. famata, C. albicans, … | +(neurodeg.) | |
Directed against herpesvirus | |||
anti-cytomegalovirus | tegument protein pp65 | + |