Table 2 Summary of the results obtained with the antibodies used for immunohistochemistry.

From: Exploring the elusive composition of corpora amylacea of human brain

 

CA staining

Presence on CAb

Positive controls

secondary antibody

secondary antibody

α-IgG isotype-specific

α-IgG non-isotype-specifica

α-IgM

α-IgG isotype-specific

α-IgG non-isotype-specific

primary antibody (IgG)

GFAP

−

 

+

negative

astrocytes

 

S100

−

 

+

negative

astrocytes

 

AQP4

 

−

−

negative

 

astrocytes

NeuN

−

 

+

negative

neuronal soma

 

TUJ1

−

 

+

negative

neuronal mt.

 

HSP70

−

 

+

doubtful

not observed

 

Ubi

+

 

−

positive

dys. neurites

 

p62

+

 

−

positive

dys. neurites

 

GS

 

+

−

positive

 

polyglucosans

MMP2

 

+

−

positive

 

thin filaments

LC3B

 

+

+

doubtful

 

not observed

  1. aα-IgG non-isotype-specific secondary antibodies were used when the isotype-specific antibodies were unavailable.
  2. bPresence on CA can be assumed only when the antibody labels the expected structure (positive control) and (a) the secondary antibody is isotype-specific, or (b) the secondary is non-isotype-specific but the vial of the primary antibody is not IgM contaminated. mt.: microtubules; dys.: dystrophic.
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