Figure 3
Confirmation of insertion and complete segregation using PCRs with genomic DNA as the template from WT, WTc and the OX strains, including OX + X (A), OX + C (B) and OX + XC (C), corresponding to the physical map in Fig. 2. Lane M: GeneRulerTM DNA ladder (Fermentas). The different primer pairs was used to amplify each gene fragment as indicated in Table 3 including different 10 pairs of primers. The cropped gels (in C) were taken from the same gel cutting out the repeated bands of transformants as shown in Supplementary information.
