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Figure 1

From: Low-dose calcipotriol can elicit wound closure, anti-microbial, and anti-neoplastic effects in epidermolysis bullosa keratinocytes

Figure 1

Calcipotriol at low concentrations is not toxic to RDEB keratinocytes. (a) Two RDEB-2, RDEB-3, and normal human keratinocyte (NHK) cell lines were treated with increasing concentrations of calcipotriol (0.001–100 µM) for 72 hrs and MTT assays performed to assess cell viability. The percentage of metabolically active cells was calculated relative to DMSO-treated controls. Anti-proliferative effects were observed at concentrations of ≥1 µM calcipotriol. (b) Microscopic analysis revealed cell toxicity in all cell lines investigated using 100 µM calcipotriol after 72 hrs, whereas cells treated with 100 nM calcipotriol showed similar cell morphology to untreated controls. Scale bar: 50 µm.

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