Figure 3 | Scientific Reports

Figure 3

From: Low-dose calcipotriol can elicit wound closure, anti-microbial, and anti-neoplastic effects in epidermolysis bullosa keratinocytes

Figure 3

Calcipotriol enhances scratch closure in RDEB monolayers. (a) Basal scratch closure of NHK, RDEB-2 and RDEB3 cells were monitored over a 48-hr time period by live microscopy. Experiments were carried out in duplicates and mean ± SD of 6 independent experiments is shown. RDEB-2 keratinocytes showed significantly reduced closure rates compared to NHK (two-way ANOVA repeated measures). (b) Calcipotriol treatment of RDEB-2 cells significantly enhanced scratch closure rates by 1.9-fold after 24 and 48 hrs. The mean ± SD of 3 independent experiments is shown (unpaired Student’s t-test, two tailed). (c) Microscopic analysis of scratch closure upon treatment of RDEB-2 cells with 100 nM calcipotriol or DMSO at indicated time points. Pictures were taken using a Nikon Eclipse Ti microscope, 10x magnification.

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