Figure 3
From: Establishment of keratinocyte cell lines from human hair follicles
Primary and SVTERT epidermal KC form fully differentiated skin equivalents. Normal abdominal skin and human skin equivalents (HSE) at day seven were analyzed by hematoxylin and eosin (H&E) and immunfluorescence staining. HSE with primary and SVTERT KC developed a multilayered epidermis with intact stratum granulosum and stratum corneum resembling human skin. The differentiation associated proteins keratin 10, keratin 2, involcurin and filaggrin were detected by immunofluorescence staining, both in normal skin and HSE cultures. One representative experiment out of three is shown; Scale bar = 120 µm.
