Figure 1
From: Redox control and autoxidation of class 1, 2 and 3 phytoglobins from Arabidopsis thaliana
Preparation of pure recombinant phytoglobins. SDS-PAGE showing the TEV mediated His8-MBP removal for AtHb1 and AtHb2 (a) and final anion exchange purification of AtHb3 (b). ‘MBP’ stands for cleaved recombinant His8-MBP affinity tag and ‘TEV’ for recombinant His6-TEV protease. Corresponding full-length gels are shown in Figure S4.
