Figure 3 | Scientific Reports

Figure 3

From: Reconfiguration of the plastid genome in Lamprocapnos spectabilis: IR boundary shifting, inversion, and intraspecific variation

Figure 3

Analysis of plastome arrangements in Lamprocapnos. (A) PCR strategy for detecting inversions and translocations. Arrowheads with red lines on the inside or outside of the circle indicate the position and orientation of the PCR primers used to confirm the structure of the plastid genome. (B) Assay results using primers designed to amplify 11 regions. Lane M contains the SolGentTM 1 kb plus DNA ladder, and lanes (1–11) correspond to the numbers of the 11 PCR amplicons in (A) or (C). The full-length electrophoretic gel is presented in Supplementary Fig. 12. (C) Linear plastome map of Lamprocapnos with the 11 PCR amplicon sets. The genes above and below the horizontal line correspond to the genes in Fig. 1. (D) Graph showing the base per base depth of the sequencing coverage across the Lamprocapnos plastome with one IR region. The red dot-dashed line indicates the coverage of the plastome with two IRs.

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