Figure 6 | Scientific Reports

Figure 6

From: Differential effects of spinal motor neuron-derived and skeletal muscle-derived Rspo2 on acetylcholine receptor clustering at the neuromuscular junction

Figure 6

Quantitative RT-PCR (qRT-PCR) analysis of the diaphragms and spinal cords in wild-type (WT), Rspo2−/−, MCK-RSPO2/Rspo2−/−, and VAChT-RSPO2/Rspo2−/− embryos at E18.5. (A) qRT-PCR of genes for the NMJ (Lgr5, MuSK, and Lrp4), Wnt signaling targets (Ctnnb1, Myc, and Axin2), and myogenesis markers (Myod and Pax7) in the diaphragms. (B) qRT-PCR of genes for the SMNs (Chat and Agrn) and Wnt signaling targets (Ctnnb1, Myc, and Axin2) in the spinal cords. (A,B) mRNA levels were normalized for beta2-microglobulin (B2m) and also for wild-type mice. Mean ± SEM (n = 3 mice) are indicated. When the p-value by one-way ANOVA is less than 0.05, p-values by post-hoc Tukey test are indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.

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