Table 1 Summary for 21 Arabidopsis traits.

From: Natural variation among Arabidopsis thaliana accessions in tolerance to high magnesium supply

Traits

Full name of the trait

Unit

Trait description

RG

Days to root germination

day

The number of days from seeding until emergence, with more than half of the seedlings having a first radicle.

PR

Primary root length

cm

After 8 days of growth under the two treatment conditions, the plants were flattened directly on agar and imaged using a camera. The primary root lengths and lateral root number were determined across the median seedling using ImageJ.

LR

Lateral root number

 

SG

Days to seed germination

day

The number of days from seeding until emergence, with more than half of the seedlings having the first cotyledon.

EL

Epicotyl length

cm

After 8 days of growth under the two treatment conditions, the plants were flattened directly on agar and imaged using a camera. The epicotyl length and rosette width were determined across the median seedling using ImageJ.

RW

Rosette width

cm

FW

Fresh weight of plants

mg

All fully-expanded seedlings without lesions were collected from each of 4 plants for each accession and weighed to obtain the fresh weight. The results are the average value across all available replicates.

K-A

The amount of potassium absorbed per plant

mg K/plant

Elemental analysis was performed with an ICP-MS (Agilent 7500a, USA). All samples were normalized to calculated weights as previously described. The ‘Nutrient absorption(A)’ was expressed as total amount of nutrient per plant; ‘Nutrient concentration (C)’ was calculated by total amount of nutrient per plant /plant fresh weight (FW). The results are the average value across all available replicates.

K-C

Potassium concentration per fresh weight

mg K/g (FW)

Ca-A

The amount of calcium absorbed per plant

mg Ca/plant

Ca-C

Calcium concentration per fresh weight

mg Ca/g (FW)

Mg-A

The amount of magnesium absorbed per plant

mg Mg/plant

Mg-C

Magnesium concentration per fresh weight

mg Mg/g (FW)

S-A

The amount of sulfur absorbed per plant

mg S/plant

S-C

Sulfur concentration per fresh weight

mg S/g (FW)

Fe-A

The amount of iron absorbed per plant

mg Fe/plant

Fe-C

Iron concentration per fresh weight

mg Fe/g (FW)

Mn-A

The amount of manganese absorbed per plant

mg Mn/plant

Mn-C

Manganese concentration per fresh weight

mg Mn/g (FW)

Na-A

The amount of sodium absorbed per plant

mg Na/plant

Na-C

Sodium concentration per fresh weight

mg Na/g (FW)