Figure 1
QB-90 adjuvant activity is independent of antigen-adjuvant mixture, but these should be administered in the same site. Female CF1 mice (n = 5) were subcutaneously immunized in the hind limb twice (0 and 14 day) at two weeks intervals with BVDV antigen and QB-90. Groups were inoculated with QB-90 and with BVDV antigen separately, either in the same or opposite sites, and at the same time or with different time points (BVDV antigen was inoculated simultaneously or 24 or 48 hours after the QB-90 inoculation). In addition, a group of mice was inoculated with the formulated vaccine (containing BVDV antigen and QB-90 as adjuvant). IgG1 (A) and IgG2a (B) anti-BVDV antibodies titers and DTH responses (C) at day 28 are shown in box-and-whiskers. Horizontal continuous lines represent median values in mice inoculated with BVDV alone, and dashed lines represent maximum and minimum values in the same group. Statistical analyses were performed using Kruskal-Wallis test (*** denotes p < 0.001, in relation to animals immunized with BVDV alone).
