Figure 1
(a) Experimental plan for dosage, timing, and location of injection animals. (b) Logarithmic VRMS amplitude of Tibialis Anterior (TA) motor activity in response to 473 nm, 45 mW/mm2 transdermal illumination of the proximal tibia for 4 s at 5 Hz and 10 ms PW for different groups. (2wk = 2 week, P2 = 2 day postpartum, s.n. = Sciatic Nerve) (c) Biodistribution results for 3E12 vp and 3E10 vp injected animals. (GN = Gastrocnemius Muscle, Nerve = Sciatic Nerve). n = 3 biologically independent samples for each group. (d) Time-course for Rat #1 in group TA + s.n. P2 & 2 wk. This animal did not lose transdermal expression up through 72 weeks post-injection, when rat was euthanized. Logarithmic VRMS activity at the sciatic and peroneal nerves at time of euthanasia with different illumination sources shown, as well as sciatic nerve cross-section showing ChR2+ axons (green) and DAPI (blue): scale bar = 30 µm.
