Figure 2
(a) Coronal lumbar H&E spinal cord section of ChR2-EYFP+ neurons in rat showing ipsilateral inflammation present. Scale bar = 80 μm. Experiment repeated 4 times with similar results. (b) Lumbar spinal cord cross-section immunohistochemistry for ChR2-EYFP (red), CD8 (brown), and hematoxylin (blue). Scale bar = 60 µm. Experiment repeated 3 times with similar results. (c) Sciatic nerve (R) and contralateral sciatic nerve (L) stained for ChR2-EYFP (green), CD8 (red), DAPI (blue), and background axons (magenta) with tibial (t.n.) and common peroneal (c.p.n.) nerve divisions labeled. Scale bar (R) = 250 µm; Scale bar (L) = 500 µm. Experiment repeated 2 times with similar results. (d) Lumbar spinal cord cross-section immunohistochemistry for ChR2-EYFP (red), CD68 (brown), and hematoxylin (blue). Scale bar = 60 µm. Experiment repeated 3 times with similar results. (e) Tibialis Anterior (TA) cross-section of ChR2-transduced rat showing myocytes which are healthy (arrowhead) and those with denervation atrophy (arrow): Scale bar = 60 µm. Experiment repeated 3 times with similar results. (f) Ventral root cross-section immunohistochemistry for ChR2-EYFP (red), CD68 (brown), and hematoxylin (blue). Scale bar = 20 µm. Experiment repeated 2 times with similar results.
