Figure 5
(a) Tibialis Anterior (TA) EMG recordings in response to 473 nm transdermal illumination of proximal tibia in rats transduced with rAAV-ChR2 restricted by either hSyn or CAG promoter. (b) Logarithmic minimum transdermal illumination power needed to elicit transdermal EMG spikes from TA for hSyn and CAG promoters: VRMS spike threshold set to 2.45 µV, which was empirically determined to be the max noise level of recordings. (c) TA cross-section of CAG rat 8 weeks post-injection showing healthy myocytes devoid of inflammation on contralateral limb (left) and ChR2-EYFP transduced myocytes co-localized with significant inflammatory cells on ipsilateral limb (red, right): scale bar = 40 µm. Experiment repeated 1 time with similar results. (d) Immunofluorescence for ChR2+ axons in CAG-ChR2-EYFP sciatic nerve sections along with counts in peroneal (c.p.n.) and tibial (t.n.) sections: scale bar = 20 µm. Experiment repeated 1 time with similar results.
