Figure 2 | Scientific Reports

Figure 2

From: Permissiveness to form pluripotent stem cells may be an evolutionarily derived characteristic in Mus musculus

Figure 2

The non-permissive NOD/ShiLtJ phenotype is recessive to permissive 129S1/SvImJ. (A,D) Emerging iPSC were three-dimensional ESC-like colonies. (B,E) F1 iPSC were expanded as single cells using trypsin. (C,F) The cells were stained for the ESC marker PECAM1 (green) and the EpiSC maker CD40 (red). The F1 colonies stained homogenously for the ESC marker PECAM1 and did not stain for the EpiSC CD40 marker. (G) iPSC generated from an F1 cross between the NOD/ShiLtJ and WSB/EiJ strains were flat and showed signs of differentiation. (H) WSB/EiJ x NOD/ShiLtJ were passaged with trypsin. The colonies could not be expanded as single cells with trypsin and showed signs of differentiation and cell death. (I) WSB/EiJ x NOD/ShiLtJ iPSC developed three-dimensional dome shaped ESC-like colonies when passaged as cell clumps using collagenase type IV and grown on a MEF feeder layer. (J) WSB/EiJ x NOD/ShiLtJ iPSC maintained on gelatin developed flattened colonies, where individual spindle-like cells could be distinguished. Scale bars represent 200 μm.

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