Figure 5 | Scientific Reports

Figure 5

From: Permissiveness to form pluripotent stem cells may be an evolutionarily derived characteristic in Mus musculus

Figure 5

iPSC were more similar to the ESC state than to the EpiSC state. The tables list the iPSC, ESC, and EpiSC samples included in RNA sequencing. (A) Only the NOD/ShiLtJ male iPSC were excluded from analysis. (B) The NOD/ShiLtJ male iPSC and the WSB/EiJ ESC were excluded from analysis. For both tables, ESC are the first column, iPSC are the second column, and EpiSC are the third column. NOD/ShiLtJ and WSB/EiJ iPSC grown in 2iS more closely resembled ESC than EpiSC (in culture row). They stained homogeneously for the ESC marker PECAM1 (green) and did not stain for the EpiSC marker CD40 (red) (cell surface staining row). Lastly, there were fewer differentially expressed genes between iPSC and ESC than iPSC and EpiSC, indicating that iPSC-derived from NOD/ShiLtJ and WSB/EiJ grown in 2iS are transcriptionally closer to the ESC cell state (RNA analysis row). Scale bars represent 200 μm.

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