Figure 3 | Scientific Reports

Figure 3

From: Development of a novel Hsp90 inhibitor NCT-50 as a potential anticancer agent for the treatment of non-small cell lung cancer

Figure 3

Association of apoptosis with NCT-50-induced cell death. (a–d) H1299 and H460 cells were treated with NCT-50 for 2 days. (a) The distribution of cells in each phase of the cell cycle was analyzed by flow cytometry. (b) Condensed, fragmented or degraded nuclei was analyzed by Hoechst 33258 staining and counted. (c) The level of cleaved PARP expression was analyzed by Western blot analysis. (d) The annexin V-positive cell population was determined by flow cytometry as described in Methods. The bars represent the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 by Student’s t-test compared with vehicle-treated control group.

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