Figure 4
Effect of PDS on the thermal stabilization and folding of G4. (A) FRET-melting curves of ssDNAs after annealing and incubation with various amounts of PDS (indicated by the coloured curves). The dashed line indicates the median value between the minimum and maximum fluorescence. (B) WT ssDNA was subjected to the Taq polymerase assay in the presence of various concentrations of PDS. The quantification of stop bands is shown in the right panel. (C) Double-stranded mutated (Mut-D) DNAs were subjected to annealing after the addition of various amounts of PDS, and then the formed G4s were detected by native PAGE analysis. The *symbol denotes an erratic product.
