Figure 5

EMSA results showing the binding of MBP-tagged Sp1 to dsDNA with or without G4. (A) WT-D subjected to annealing, (B) WT-D without annealing, and (C) Mut-D subjected to annealing were incubated with the indicated amounts of Sp1, and then the samples were subjected to native PAGE analyses. The dotted box denoted the Sp1 and DNA complex. The quantification of unbound DNA is shown in the right panel.