Figure 5

mDSLM mitigates shadowing artifacts and enables contrast-enhanced imaging in biological tissues. SPIM, mSPIM, DSLM, and mDSLM images of human breast tissue stained with eosin are shown in (a). Shadowing artifacts are visible in the SPIM and DSLM images, whereas reduced contrast is observed in the SPIM and mSPIM images. Representative zoomed-in views demonstrating the mitigation of shadowing artifacts for mDSLM versus DSLM, and enhanced imaging contrast for mDSLM versus mSPIM, are shown in (b). Intensity line profiles through the zoomed-in views in (b) are plotted in (c). The dashed lines in (c) mark the y positions of the shadows seen in the DSLM image. SPIM, mSPIM, DSLM, and mDSLM images of murine small intestine, stained with acridine orange, are shown in (d). En face images in the xy plane are shown in (e) at z positions (depths) of 100, 250, 400, and 550 μm. Intensity line profiles along the dashed line in the images of the crypts at z = 550 μm are plotted in (f), with values for image contrast shown on the right. The dashed lines in (f) mark the y positions of the shadows seen in the DSLM images in (d,e), and which are mitigated in the mDSLM images.