Figure 2
From: Fibulin-2 is required for basement membrane integrity of mammary epithelium
Size increase of Matrigel-embedded EpH4 cells associated with FBLN2 KD. (a) Phase contrast microscopy of the stably transduced EpH4 cells grown in Matrigel matrix expressing scr vector, shRNA#1, shRNA#2, or shRNA#3, showing the size increase in spheroids upon Fbln2 KD. Pictures are representative of three independent experiments. Scale bars represent 500 µm (top) and 100 µm (bottom). (b) Quantification of small and large spheroidal structures of stably transduced EpH4 cells grown in Matrigel matrix. Bars are standard error for three biological replicates.
