Figure 4
From: Fibulin-2 is required for basement membrane integrity of mammary epithelium
Fbln2 KD is coupled with a reduction in Integrin beta 1 expression but not Integrin alpha 3. (a) Immunofluorescent analysis of FBLN2, ITGβ1 and ITGα3 expression in the stable KD cell lines with the lowest (18%) FBLN2 expression. Bars represent 50 μm (b) Western blotting analysis of ITGα3 and ITGβ1 relative to actin in protein extracts from 2D-plated scr ctrl and Fbln2 KD cells. (Note: The ITGα3 was measured on the same membrane as FBLN2 in Fig. 1a after antibody stripping; ITGβ1 and actin are from the same blot, though from equally loaded neighbouring parts of the gel).
