Figure 2 | Scientific Reports

Figure 2

From: Comparative in depth RNA sequencing of P. tricornutum’s morphotypes reveals specific features of the oval morphotype

Figure 2

Summary of the experimental design and analytical pipeline used in this study. Briefly, 4 biological replicates of cultures enriched for each specific morphotype were performed in parallel. The RNA were independently purified and used as a matrix to build libraries for RNA sequencing. Quality of the raw data generated have been checked with FastQC. Then, the short reads were aligned on the annotated genome with TopHat2. Aligned reads have been counted with HTseq DESeq2 have been used to normalize counts and to perform the differential analysis. Finally, GO enrichment have been done with B2GO for functional analysis.

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