Figure 5

Expression levels of XvE93, XvHR3, XvE75, and XvBr-C in the cephalothorax and abdomen of Xenos vesparum females. (A) Dorsal views of a 4th instar larva and a neotenic adult female. The abdomen (abd.) and the cephalothorax (cep.) regions are marked. (B–E) Transcript levels of (B) XvE93, (C) XvHR3, (D) XvE75, and (E) XvBr-C (without discriminating isoforms) were measured by qRT-PCR in the cephalothorax and abdomen of 4th instar larvae and neotenic adult females. Transcript abundance values in panels B-E are normalized against the XvRibosomal 18S transcript. The range of expression is shown by Boxplot: boxes demarcate the upper and lower quartiles, while the heavy bar indicates the median value of normalized expression. Whiskers extend to the most extreme values. Points represent each individual measurement. Asterisks indicate differences statistically significant at *p ≤ 0.05; **p ≤ 0.01; and ***p ≤ 0.001 (t-test).