Figure 6

Developmental expression profiles of Xenos vesparum XvKr-h1 in the larva to adult transition. (A) XvKr-h1 mRNA levels were measured by qRT-PCR in the larva-to-adult transition in male (4^th, pupa, adult) and female (4^th and adult) whole bodies. The inset panel shows a magnification of expression levels in 4^th larval instar and pupa. (B) XvKr-h1 mRNA levels (qRT-PCR) in the whole body of 3^th, early and late 4^th and neotenic females. (C) XvKr-h1 mRNA levels (qRT-PCR) in the cephalothorax (cep.) and abdomen (abd.) of 4^th instar larvae and neotenic adult females. Transcript abundance values in all panels are normalized against the XvRibosomal 18S transcript. The range of expression is shown by Boxplot: boxes demarcate the upper and lower quartiles, while the heavy bar indicates the median value of normalized expression. Whiskers extend to the most extreme values. Points represent each individual measurement. Different letters in panel B represent groups with significant differences according to ANOVA test (Tukey, p ≤ 0.005). Asterisks in panel C indicate differences statistically significant at **p ≤ 0.01 (t-test).