Figure 2
From: Identification of RSPO2 Fusion Mutations and Target Therapy Using a Porcupine Inhibitor
The effect of EMC2-RSPO2 fusion gene expression in HEK293 cells. (a) Western Blot assay of LGR5 protein in HEK293 and LoVo cells. (b) WNT/β-catenin pathway activities in HEK293- TCF cells transfected with pcDNA-R2, pcDNA-ER2 and pcDNA3.1 plasmid. (c) β-catenin expression by q-PCR detection in HEK293 after transfection of pcDNA-R2, pcDNA-ER2 and pcDNA3.1. (d) RSPO2 protein expression in HEK293 after transfection of pcDNA-R2, pcDNA-ER2 and pcDNA3. (e) Cell proliferation curves of HEK293 stably transfected by plasmid pcDNA-R2, pcDNA-ER2 and pcDNA3. (f) Tumor growth curves by the HEK293-pER2, HEK293-pR2, HEK293-pcDNA3.1 induced by implanting 5×106 cells in nude mice. Mice number N = 10. *p < 0.05, **p < 0.01, ***p < 0.001.
