Figure 3 | Scientific Reports

Figure 3

From: Site-1 protease function is essential for the generation of antibody secreting cells and reprogramming for secretory activity

Figure 3

Kinetic and dose analysis of S1P-specific inhibitor PF-429242 during the early phase of ASC generation. (A) In vitro activated human B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) at day 3 and assessed for CREB3L2 processing at 12 h intervals. Representative results are shown from a total of 7 donors. (B) Samples from part (A) were assessed for cell number at the indicated time points. Displayed are the average cell number ± standard deviation derived from 7 donors. (C) In vitro activated human B-cells from 4 donors were treated with indicated amount PF-429242 or vehicle control (dH2O) at day 3 and assessed for cell number at 48 h. Results are displayed as the percentage viable cells relative to control.

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