Figure 4 | Scientific Reports

Figure 4

From: Site-1 protease function is essential for the generation of antibody secreting cells and reprogramming for secretory activity

Figure 4

S1P inhibition induces autophagy and affects multiple UPR-related TFs. (A) In vitro day 3 B-cells were treated with 10 μM PF-429242 or vehicle control (dH2O) for 72 h and were assessed for cell number using CountBright beads. Shown are the average cell numbers from 7 donors ± standard deviations. Protein lysates generated from day 3 cells treated for 72 h with 10 μM PF-429242 were evaluated for (B) autophagy markers or (C) CREB3L2 and ATF6 by Western blotting. Shown are representative results from 2 donors, a total of 7 donors were evaluated.

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