Figure 2
Homotaurine treatment at time of clinical EAE onset reduces the frequency of autoreactive IL-17A- and IFNγ-secreting T cell responses, while increasing IL-10-secreting responses. Mice were PLP139–151 immunized and after reaching an EAE score of 1, they received plain water or homotaurine for five days. Splenic T cells were isolated from individual mice and the percentages of IL-17A, IFNγ and IL-10 secreting T cells responding to PLP139–151 were measured by ELISPOT. Data shown are the mean SFC ± SEM of each group of mice (n = 5 per group) from two separate experiments. IL-4 responses to PLP139–151 were at background levels (data not shown). Wells containing cells from control or homotaurine-treated mice that were incubated with medium alone had 0–5 SFC. Pairwise comparisons were performed by 2-tailed Student’s t test. **p < 0.01 vs. the controls.
