Figure 3
Altered proportions of intermediate and non-classical monocyte subsets are associated with differential mycobacterial control. Monocyte subsets were characterized among patients with active TB disease (n = 17), LTBI (n = 17) and healthy controls (n = 10) before starting anti-TB treatment. The proportions of classical (A), intermediate (B), and non-classical (C) monocytes were calculated from the total monocyte population. A negative correlation between intermediate monocytes and net growth (D), and a positive correlation between non-classical monocytes and net growth (E) of BCG were seen. The latter was also seen with M.tb H37Rv (data not shown). Monocyte subsets were then characterized after completion of treatment among active TB (n = 11) and LTBI (n = 14) patients. The proportions of intermediate (F) and non-classical (G) monocytes are shown. Points are single values and bars represent the mean with SD. After testing for normality, an ordinary one-way ANOVA with Tukey’s correction (A,C,G) or a Kruskal-Wallis test with Dunn’s correction for multiple comparisons (B,F) was performed. *Represents a p-value of <0.05, **a p-value of <0.005, ***a p-value of <0.0005 and ****a p-value of <0.0001. For correlations Spearman’s rho and associated p-values are shown.
