Figure 4
EC nestin is regulated by laminar shear stress in vitro. Immunofluorescence staining of nestin in HUVEC, HDMEC, HCAEC and HPAEC cultured under (A) static conditions, or (B) 10 dyne/cm2 laminar shear stress for 24 hours. Quantification of nestin spatial distribution in (C) HUVEC, (D) HDMEC, (E) HCAEC and (F) HPAEC. Each point represents an individual cell (n = 3–19 different experiments) Unpaired t-test *p-value < 0.05, **<0.01 ****<0.0001. (G) HUVEC were cultured under 10 dyne/cm2 laminar shear stress for 0 h, 1 h, 4 h or 24 h, and stained for nestin, vimentin, actin, and DAPI. Pearson’s co-localisation value for nestin and vimentin is displayed on the top right of the merged images. (H) Quantification of nestin spatial distribution over the time course (n = 6); significance was calculated using one-way ANOVA. (I) NES and (J) VIM mRNA expression in EC under both static conditions and laminar shear stress, displayed relative to 18 s rRNA (n = 6–10). All graphs show means ± SD.
