Figure 5
From: The Role of p53 in Determining Mitochondrial Adaptations to Endurance Training in Skeletal Muscle
Autophagy protein response and regulators of p53 expression in the absence of p53 and with training. (A) Proteins in the autophagy pathway were measured; (B) LC3-II/LC3-I ratio (n = 6–8/group); *p ≤ 0.05, **p ≤ 0.01, UT vs. T, 2-way ANOVA, (C) p62 (n = 5–8/group), *p ≤ 0.05, UT vs. T; ††p ≤ 0.01, UT WT vs. mKO, 2-way ANOVA, (D) Parkin (n = 5–7/group); *p ≤ 0.05, UT vs. T, Student’s t-test and 2-way ANOVA; ††p ≤ 0.01, UT WT vs. mKO, 2-way ANOVA, and (E) Beclin-1 (n = 5–7/group); *p ≤ 0.05, UT vs. T, Student’s t-test; ††p ≤ 0.01, UT WT vs. mKO, 2-way ANOVA. (F) Regulators of p53 expression (Mdm2) and its mitochondrial localization (CHCHD4) were next examined. Whole muscle protein expression of (G) Mdm2 (n = 5–6/group); *p ≤ 0.05, **p ≤ 0.01, UT vs. T, 2-way ANOVA, and (H) CHCHD4 (n = 5–6/group); **p ≤ 0.01, UT vs. T, Student’s t-test and 2-way ANOVA; ††p ≤ 0.01, UT WT vs. mKO, Student’s t-test, was measured. Data are presented as mean ± SEM.
