Table 2 Strains, plasmids and primers used in this study.
Strains, plasmids or primers | Characteristics | Sources or references |
|---|---|---|
Strains | ||
Yb2 | Riemerella anatipestifer serotype 2 strain | |
Yb2ΔbioF | Tn4351 insertion mutant of R. anatipestiferYb2, bioF::Tn | |
cYb2ΔbioF | Mutant strain RA1893 carrying plasmid pCP29-bioF | This study |
Escherichia coli S17-1 | λpirhsdRprothi; chromosomally integrated RP4-2, Tc::Mu Km::Tn7 | |
Plasmids | ||
pCP29 | ColE1 ori (pCP1 ori); Apr(Emr); E. coli-F. johnsoniae shuttle plasmid | |
pCP29-bioF | pCP29 containing ompA promoter and bioF ORF, cfxAr(Apr) | This study |
Primers | ||
ompA promoter P1 | 5′-CAGGTACCATAGCTAAAATTTTGGCAGTAAC-3′ (KpnΙ site underlined) | |
ompA promoter P2 | 5′-CGACTCGAGCATTCCAATTCTCTTATTATC-3′ (XhoΙ site underlined) | |
16S rRNA F | 5′-GAGCGGTAGAGTATCTTCGGATACT-3′ | |
16S rRNA R | 5′-AATTCCTTTGAGTTTCAACCTTGCG-3′ | |
bioF comp-F | 5′-CCGCTCGAGATGAATATTCCCAAAAGATTGTTAGAT-3′ (XhoΙ site underlined) | This study |
bioF comp-R | 5′-CATGCATGCTTATTTCTTTAAATTAGAAATGATAAAATTGTGGGC-3′ (SphΙ site underlined) | This study |
