Figure 2 | Scientific Reports

Figure 2

From: Increased TIMP-3 expression alters the cellular secretome through dual inhibition of the metalloprotease ADAM10 and ligand-binding of the LRP-1 receptor

Figure 2

Validation of TIMP3/HEK secretome analysis by immunoblotting. (A,B) Immunoblots (A) and their respective quantification (B) showing the expression of TIMP-3, sAPP, sAPPα in the conditioned media of TIMP3/HEK and control HEK293 cells, and full length APP and actin in cell lysates (densitometric quantifications shown as mean values ± standard deviation; *p < 0.05, **p < 0.01, ***p < 0.005, Student’s t-test; 3 independent experiments are displayed out of 6 quantified, n = 6). C-D. Immunoblots (C) and their respective quantifications (D) showing the decrease of shed EphA4 (sEphA4) in the conditioned media of HEK cells cultured in the presence of TAPI or GI254023X (densitometric quantifications shown as mean values ± standard deviation; *p < 0.05, **p < 0.01, ***p < 0.005, Student’s t-test; 3 independent experiments are displayed out of 6 quantified, n = 6). (E,F) Immunoblots (C) and their respective quantification (D) showing the increase of TIMP-1, MIF and SPARC in the conditioned media of TIMP-3 expressing cells (densitometric quantifications shown as mean values ± standard deviation; *p < 0.05, **p < 0.01, ***p < 0.005, Student’s t-test; 3 independent experiments are displayed out of 6 quantified, n = 6). MMP-1 is shown as an example of a protein that does not increase in the conditioned media of TIMP-3/HEK293.

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