Figure 4

TIMP-3 competes with TIMP-1 for its binding to LRP-1. (A) Full-length LRP1 was coated onto microtiter plates and binding of FLAG-tagged TIMP-1 (0–48 nM) was measured using an M2 anti-FLAG antibody (n = 3). (B) Similarly, binding of 24 nM FLAG-tagged TIMP-1 to LRP-1 was measured in the absence (control) or presence of 2 nM TIMP-3.