Figure 1
Effects of culture media on activation of limbal stem/progenitor cells in limbal explant outgrowth sheets. (A) Representative phase contrast micrographs of limbal explant and outgrowths in culture medium complemented with (I) 5% FBS with CT, (II) 5% FBS without CT, and (III) 5% human serum without CT using transwell (without; W/O amniotic membrane) and amniotic membrane. Bar = 50 µm. (B) Representative phase contrast micrographs of holoclone colonies from outgrown cells from limbal explant (upper). Photographs of colonies stained with Coomassie blue R solution (lower) and CFE ratio. (C) Representative JC1 staining bivariate plots of limbal explant outgrowth cultures with JC1 exclusion cell cohort (JC1low) indicated. Mean ± SD for three independent experiments are shown within the plots. (D) Representative western blot image on p63α and ABCG2 protein expression. All signal intensities were normalized to the signal generated in the same sample by β-actin. *p < 0.05, **p < 0.01 (n = 6 from 3 donors).
