Figure 2
Limbal explant outgrowth sheets on substrate free, HAMTW, and HAMS. (A) Representative phase contrast micrographs of limbal explant outgrowth sheets on days 6 and 9 in TW, HAMTW, and HAMS. Bar = 50 µm. Mean ± SD for six independent experiments from three donors are shown. (B) Area of limbal explant outgrowth sheets by day 12. (C) Cell yields after culture for 12 days. (D) Ultrastructure of limbal explant outgrowth sheets using transmission electron microscopy. The multi-layers (red insert) shown on top were observed under different magnifications on the bottom, respectively. The red stars demonstrated cell-cell junction spaces which were magnified on the top right of the pictures (bottom). Bar = 2 µm (upper) and 0.2 µm (lower). (E) Representative images of explant outgrowths stained for Ki67 and graph for Ki67 expressions. Bar = 20 µm. *p < 0.05, **p < 0.01 (n = 6 from 3 donors).
