Figure 4
From: LINCS L1000 dataset-based repositioning of CGP-60474 as a highly potent anti-endotoxemic agent
Inhibitory effects of CGP-604474 on NF-κB nuclear translocation following LPS or Poly (I:C) administration in vitro. (A) The expression of NF-κB p50 and p65 by treatment with CGP-60474 was examined in 100 ng/ml LPS treated J774.1 cells. (B) The expression of NF-κB p50 and p65 by treatment with CGP-60474 was examined in 10 μg/ml poly(I:C) treated J774.1 cells. (C,D) The results of NF-κB subunit p50 quantification show that both CGP-60474 treated LPS or poly(I:C) group significantly reduced p50 nuclear localized cells. Bars, 50 μm Data are presented as the mean ± SEM; Experiment was independently repeated three times, **p < 0.01, *p < 0.05.
