Figure 1
Pipeline for identifying goat AS events in this study. Fetus, M2, Y1, and Adult represent goats at embryonic stages beyond 135 d, two months of age, one year of age, and two years of age, respectively. Heart, Kidney, Leg muscle, Liver, and Spleen with three replicates (R1, R2, and R3) at each developmental stage were collected. After filtering and merging of raw reads, and assembling of filtered reads, assembled transcripts were obtained. After AS identification based on the assembled transcripts, coding capability assessment, and identification of tissues and developmental stage specific AS was performed. Gene ontology enrichment analysis for tissues and developmental stage specific AS were performed to identify the functional roles of AS genes.
