Figure 4
From: Two Engineered OBPs with opposite temperature-dependent affinities towards 1-aminoanthracene
Fluorescence-binding assay of 1-AMA to tOBP and OBP::GQ20::SP-DS3. (a) Experimental layout for competitive binding evaluation; (b) visualization of 1-AMA in each compartment after variation of temperature; (c) amount of 1-AMA in each compartment after variation of temperature. Values are the mean ± SD of 2 independent experiments.
