Table 1 Quantitative analysis of binding interaction between CHP3 and CBD region of NHE1 by thermophoresis.

From: Calcineurin B homologous protein 3 binds with high affinity to the CHP binding domain of the human sodium/proton exchanger NHE1

sample

KD in nM

Bound (Fnorm ‰)

Unbound (Fnorm ‰)

Target conc. in nM

SD (KD) of five technical replicates per sample in nM

KD confidence interval in nM

1

62.7

882.9

916.0

10

0.9

 ± 5.2

2

54.8

879.4

916.4

10

1.0

 ± 5.4

3

49.0

880.0

905.5

10

0.8

 ± 5.3

\(\bar{{\rm{x}}}\) ± SD

55.5 ± 6.8

     
  1. \(\bar{{\rm{x}}}\) ± SD = mean of three biological replicates ± standard deviation.
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