Figure 2

Reporter probes fused with a carbohydrate binding module significantly limits diffusion across. The reporter probes were spotted (1 mL) onto the center of the filter until the entire filter was saturated by diffusion. The effect of the carbohydrate binding module (CBM) fusion to the reporter enzymes was visualized by the degree of diffusion from the center of the filter (top row). The binding strength was further evaluated by washing the filter with PBS before substrate addition (bottom row).