Figure 3
Comparison of intensities derived from labeled proteins in synaptosomes and neuronal synapses. (a) Absolute intensities (in arbitrary units) of the 10 labeled proteins in synaptosome preparations and cultured hippocampal neurons, expressed as mean values derived from all selected synapses (after final filtering). The right panel shows example synapses, marked with the respective intensities for the synapsin 1/2 stainings (in arbitrary units). Scale bar = 5 µm. (b) Average synaptic protein copy numbers, obtained by dividing the synapse intensities by the average synaptosome intensities, and multiplying with the average copy numbers per synaptosome from9. Example synapses with the respective synapsin 1/2 copy numbers are shown in the right panel. Scale bar = 5 µm. In contrast to the graph in (a), the graph in (b) is shown in log scale. Bar graphs represent mean values with standard errors of the mean (SEM). N = 875 synaptosomes (for synaptophysin and bassoon), 24–184 synaptosomes (for all other POI’s), 1809 cultured synapses (for synaptophysin and bassoon), and 51–354 synapses (for all other POI’s).
