Figure 5
Numbers of single antibodies as a measure for relative immunostaining intensity (a) Confocal and STED images of hippocampal neuron synapses and single antibodies bound to a glass coverslip. Synapses were stained for synaptophysin, bassoon and synapsin1/2 (confocal image). Super-resolution images of the signal derived from the anti-synapsin antibodies were taken in synapses (top right) or in a single-antibody configuration (bottom panels; left: scaled identically to synapses; right: autoscaled to visualize the signal of single antibodies). Scale bar = 500 nm (b) 2-dimensional scatter plot comparing numbers of antibodies per synapse derived from the measurement described in a), along the Y axis, or derived by calculation from synapse/synaptosome ratio (taken from results in Fig. 3), along the X axis. The spots represent mean values, and error bars represent the standard error of the mean (SEM). N = 632, 660, 1034 and 1009 synapses for synapsin, synaptogyrin, syntaxin 12/13 and vGlut 1/2 respectively, from two independent experiments, for the measured data. The estimated data are extracted from Fig. 3, and therefore have the same N values. The black line represents a linear regression with a slope of 1.34 and R2 of 0.955.
