Table 1 Numbers of primary antibodies per synaptosome for over 100 targets.

From: Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins

Target protein

Avergae # of ab’s

SEM/ROV

Average # of protein copies*

SEM

Company

Cat number

Species

Dilution

Actin

23.615

1.817 (SEM)

22074.6

1909.62

Novus Biologicals

NB600-535

mouse monoclonal

1:500

ADAM22

20.238

0.864 (SEM)

***

 

Novus Biologicals

NBP2-22425

mouse monoclonal

1:500

AKT (PKB)

10.326

0.239 (SEM)

***

 

Cell Signaling

4691

rabbit monoclonal

1:400

Alpha-SNAP

16.216

1.475 (SEM)

1150.7

46.62

Synaptic Systems

112 111

mouse monoclonal

1:200

Amphiphysin

19.001

1.973 (SEM)

1194.2

60.04

Synaptic Systems

120 002

rabbit polyclonal

1:500

AP180

15.174

1.139 (SEM)

3736.4

207.63

Synaptic Systems

155 003

rabbit polyclonal

1:500

AP2

19.136

1.546 (SEM)

2324.7

81.99

Abcam

ab75995

rabbit monoclonal

1:500

APP

14.186

2.776 (SEM)

6283.6

584.51

Millipore

MAB348

mouse monoclonal

1:100

Arc

18.419

0.545 (SEM)

***

 

Synaptic Systems

156 003

rabbit polyclonal

1:1000

BACE1

17.660

0.886 (SEM)

115.84

2.75

Santa Cruz

M-83

rabbit polyclonal

1:100

Bassoon

60.331

6.221 (ROV)

446.14

37.71

Enzo

ADI-VAM-PS003-F

mouse monoclonal

1:100

Bassoon

27.538

2.626 (ROV)

446.14

37.71

Synaptic Systems

141 002

rabbit polyclonal

1:500

Calbindin

33.403

0.917 (ROV)

296.88

13.22

Swant

CB38a

rabbit polyclonal

1:500

Calcineurin

11.718

0.188 (SEM)

138.67 (isoform B)

91.82

Synaptic Systems

387 002

rabbit polyclonal

1:1000

Calmodulin

27.019

4.493 (SEM)

8659.9

445.47

Novus Biologicals

NB110-55649

rabbit monoclonal

1:500

Calmodulin

13.510

1.0193 (ROV)

8659.9

445.47

Abcam

ab45689

rabbit monoclonal

1:100

Calretinin

16.688

2.724 (ROV)

369.24

2.47

Swant

CR7699/4

rabbit polyclonal

1:500

CAPS1

46.108

12.0256 (ROV)

196.42

28.58

Abcam

ab32014

mouse monoclonal

1:1000

CAPS1

23.302

2.013 (SEM)

196.42

28.58

Abcam

ab69797

rabbit polyclonal

1:500

CB1 (Anti-Cannabinoid Receptor)

12.842

0.745 (ROV)

***

 

Abcam

ab23703

rabbit polyclonal

1:50

CDC42

13.380

0.479 (SEM)

800.4

268.073

Thermo Scientific

PA1-092

rabbit polyclonal

1:100

ChromograninA

8.852

0.276 (ROV)

Presumably similar to ChromograninB

 

Synaptic Systems

259 003

rabbit polyclonal

1:500

ChromograninB

13.082

5.006 (ROV)

15.67

4.19

Synaptic Systems

259 103

rabbit polyclonal

1:500

ChromograninC

11.123

0.8085 (ROV)

Presumably similar to ChromograninB

 

Abcam

ab12241

rabbit polyclonal

1:250

Clathrin Heavy Chain

37.678

5.6 (SEM)

3472.47

174.65

BD Biosciences

610499

mouse monoclonal

1:500

Clathrin Light Chain

13.465

1.154 (ROV)

4554.06

296.74

Synaptic Systems

113 011

mouse monoclonal

1:500

Complexin1/2

15.858

2 (SEM)

2488.2

149.49

Synaptic Systems

122 002

rabbit polyclonal

1:500

CSP

16.542

3.041 (SEM)

941.18

48.86

Synaptic Systems

154 003

rabbit polyclonal

1:500

DLGAP1

31.073

4.709 (ROV)

52.961

27.765

Novus Biologicals

NBP1-76911

rabbit polyclonal

1:50

Doc2A/B

26.170

5.973 (ROV)

3696.5

164.19

Synaptic Systems

174 203

rabbit polyclonal

1:500

Dopamine ReceptorD1

16.985

1.9254 (ROV)

***

 

Abcam

ab40653

rabbit polyclonal

1:1000

Dopamine ReceptorD2

20.186

0.418 (SEM)

***

 

Merck

AB5084P

rabbit polyclonal

1:500

Drebrin1

22.188

0.532 (SEM)

342.075

85.238

Novus Biologicals

NB100-1951

mouse monoclonal

1:100

Dynamin1/2/3

17.379

1.382 (SEM)

2326.4

83.87

Synaptic Systems

115 002

rabbit polyclonal

1:500

EndophilinI

12.681

1.999 (SEM)

2524.4 (all isoforms)

67.27

Synaptic Systems

159 002

rabbit polyclonal

1:500

Epsin1

20.954

1.306 (SEM)

92.88

4.3

Novus Biologicals

EPR3023

rabbit polyclonal

1:100

ERp72 (PDIA4)

17.546

0.585 (SEM)

***

 

Cell Signaling

5033

rabbit monoclonal

1:100

GluK1 (Kainate Receptor)

15.725

2.207 (ROV)

***

 

Alomone

AGC-008

rabbit polyclonal

1:100

GluN1 (Kainate Receptor)

15.382

2.161 (SEM)

***

 

Synaptic Systems

114 011

mouse monoclonal

1:1000

GluN2A (Glutamate Receptor)

23.966

1.091 (SEM)

***

 

NeuroMab

75–288

mouse monoclonal

1:100

GluN2B (Glutamate Receptor)

8.549

1.06 (SEM)

83.6224

20.6121

NeuroMab

75–101

mouse monoclonal

1:100

GluR3 (Glutamate Receptor)

20.061

0.998 (SEM)

***

 

Invitrogen/Life Technologies

32–0400

mouse monoclonal

1:100

Homer1

9.363

1.947 (SEM)

712.817

83.691

Synaptic Systems

160 011

mouse monoclonal

1:500

Homer3

12.718

0.691 (ROV)

207.483

66.367

Synaptic Systems

160 303

rabbit polyclonal

1:250

Hsc70

24.154

3.72 (SEM)

8210.1

404.5

Santa Cruz

sc7298

mouse monoclonal

1:500

IGF-1R

10.494

0.935 (ROV)

***

 

Cell Signaling

3027

rabbit polyclonal

1:300

Intersectin1

26.077

2.955 (SEM)

3096.5

277.62

Haucke (Berlin)

 

rabbit polyclonal

1:500

Kir2.1

8.312

0.3 (ROV)

***

 

Novus Biologicals

NBP1-95482

rabbit monoclonal

1:100

Kv1.1

13.047

4.988 (ROV)

201.2956

95.671

Thermo Scientific

PA5-19593

rabbit polyclonal

1:100

Kv2.1

10.647

1.674 (ROV)

***

 

Synaptic Systems

231 002

rabbit polyclonal

1:500

Munc13a

19.037

1.047 (SEM)

1551.3

53.18

Synaptic Systems

126 102

rabbit polyclonal

1:500

Munc18a

21.108

1.53 (SEM)

4253.4

207.07

Synaptic Systems

116 002

rabbit polyclonal

1:500

Myosin5a

9.245

1.271 (SEM)

157.24

20

Sigma-Aldrich

M5062

rabbit polyclonal

1:200

NavBeta1

18.611

1.972 (SEM)

830.8294

248.9188

Alomone

ASC-041

rabbit polyclonal

1:50

nAChRBeta2

21.164

0.718 (SEM)

***

 

Alomone

ABC-012

rabbit polyclonal

1:100

NaKATPase

29.913

0.961 (SEM)

3771.7

481.7

Thermo Scientific

MA3-915

mouse monoclonal

1:1000

Nav1.1

13.934

1.261 (SEM)

***

 

Merck

06-811

rabbit polyclonal

1:100

Nav1.3

10.685

0.805 (SEM)

***

 

Alomone

ASC-004

rabbit polyclonal

1:250

nNOS

7.441

0.773 (ROV)

***

 

Thermo Scientific

PA1-033

rabbit polyclonal

1:100

NSF

15.662

0.432 (SEM)

677.45

213.02

Synaptic Systems

123 002

rabbit polyclonal

1:100

NSF

17.220

0.976 (SEM)

677.45

213.02

Synaptic Systems

123 002

rabbit polyclonal

1:500

Parvalbumin

33.327

0.435 (ROV)

681.1

34.31

Swant

PV25

rabbit polyclonal

1:500

Piccolo

32.092

0.322 (ROV)

100.45

8.4

Synaptic Systems

142 003

rabbit polyclonal

1:200

PSD95

17.742

1.163 (SEM)

1500.2

349.9

Sigma

P246

mouse monoclonal

1:200

PSD95

19.390

1.387 (SEM)

1500.2

349.9

Cell Signaling

3450

rabbit monoclonal

1:100

Rab3a

19.254

2.613 (SEM)

18846.58

996.01

Synaptic Systems

107 003

rabbit polyclonal

1:500

Rab5a

29.144

4.168 (SEM)

633.62

37.26

Cell Signaling

3547 S

rabbit polyclonal

1:500

Rab7a

21.976

2.29 (SEM)

4457.2

319.8

Santa Cruz

sc81922

rabbit polyclonal

1:100

Rab9

10.173

1.552 (ROV)

***

 

Cell Signaling

5118

rabbit polyclonal

1:100

Rapsn

15.000

1.443 (SEM)

***

 

Atlas Antibodies

HPA039475

rabbit polyclonal

1:100

Rim1

22.028

1.419 (ROV)

38.63

4.23

Synaptic Systems

140 003

rabbit polyclonal

1:200

SCAMP1

34.862

2.961 (SEM)

1459.5

115.53

Synaptic Systems

121 002

rabbit polyclonal

1:100

Sec22b

15.399

9.38 (ROV)

118.69

38.72

Synaptic Systems

186 003

rabbit polyclonal

1:100

Septin5

24.894

1.786 (SEM)

1726.2

64.38

Haucke (Berlin)

 

rabbit polyclonal

1:500

Septin7

14.637

0.168 (SEM)

2320.5

98.66

Atlas Antibodies

HPA029524

rabbit polyclonal

1:50

Shank1

10.116

0.515 (SEM)

141.276

35.11

Synaptic Systems

162 013

rabbit polyclonal

1:500

Shank2

15.660

0.638 (ROV)

168.2534

12.6241

Synaptic Systems

162 202

rabbit polyclonal

1:500

Shank3

22.504

3.638 (ROV)

181.28

53.24

Synaptic Systems

162 302

rabbit polyclonal

1:500

SNAP23

12.955

0.997 (SEM)

265.61

17.75

Synaptic Systems

111 202

rabbit polyclonal

1:100

SNAP25

10.415

2.71 (ROV)

26686.08

5287.39

Synaptic Systems

111 011

mouse monoclonal

1:100

SNAP29

19.561

2.654 (SEM)

77.47

6.47

Synaptic Systems

111 302

rabbit polyclonal

1:500

SNAP29

7.413

0.79 (ROV)

77.47

6.47

Synaptic Systems

111 302

rabbit polyclonal

1:500

SNAP47

9.079

0.546 (ROV)

~200**

 

Synaptic Systems

111 403

rabbit polyclonal

1:200

Stargazin

5.314

0.296 (SEM)

143.78

12.33

Alomone

ACC-012

rabbit polyclonal

1:200

SV2A/B

16.201

1.63 (SEM)

4616.65

128.17

Jahn Department

 

mouse monoclonal

1:100

Synapsin1/2

21.991

3.462 (SEM)

23422.77

1300.03

Synaptic Systems

106 002

rabbit polyclonal

1:500

Synaptogyrin

56.224

2.57 (SEM)

1854.8

110.49

Synaptic Systems

103 002

rabbit polyclonal

1:100

Synaptojanin

19.497

0.712 (SEM)

365.61

40.31

Synaptic Systems

145 003

rabbit polyclonal

1:100

Synaptotagmin1

15.737

1.671 (SEM)

10332

1079.2

Synaptic Systems

105 102

rabbit polyclonal

1:1000

Synaptotagmin2

15.451

1.823 (SEM)

297.28

11.37

Synaptic Systems

105 123

rabbit polyclonal

1:100

Synaptotagmin4

9.333

0.795 (ROV)

***

 

Synaptic Systems

105 143

rabbit polyclonal

1:1000

Synaptotagmin5/9

18.341

0.318 (SEM)

***

 

Synaptic Systems

105 053

rabbit polyclonal

1:100

Synaptotagmin7

29.852

1.613 (SEM)

182.64

3.54

Synaptic Systems

105 173

rabbit polyclonal

1:100

Synaptotagmin7

7.239

1.007 (ROV)

182.64

3.54

Synaptic Systems

105 173

rabbit polyclonal

1:250

Syndapin (Pacsin)

11.612

0.493 (SEM)

3201

131.28

Synaptic Systems

196 002

rabbit polyclonal

1:500

SynGAP1

7.941

1.278 (ROV)

622.07

90.73

Thermo Scientific

PA1-046

rabbit polyclonal

1:1000

Syntaxin1

16.991

1.826 (SEM)

20096

999.43

Synaptic Systems

110 011

mouse monoclonal

1:100

Syntaxin13

13.925

0.842 (SEM)

157.83

3.49

Synaptic Systems

110 131

mouse monoclonal

1:100

Syntaxin16

25.428

1.991 (SEM)

91.27

5.68

Synaptic Systems

110 162

rabbit polyclonal

1:100

Syntaxin16

12.975

0.172 (ROV)

91.27

5.68

Synaptic Systems

110 162

rabbit polyclonal

1:100

Syntaxin2

12.214

2.445 (SEM)

~100–200**

 

Synaptic Systems

110 022

rabbit polyclonal

1:100

Syntaxin3

11.433

4.084 (ROV)

<100**

 

Synaptic Systems

110 033

rabbit polyclonal

1:100

Syntaxin4

14.329

5.247 (ROV)

~100-200**

 

Synaptic Systems

110 042

rabbit polyclonal

1:100

Syntaxin5

17.280

0.66 (ROV)

~100–200**

 

Synaptic Systems

110 053

rabbit polyclonal

1:100

Syntaxin6

28.117

3.863 (SEM)

121.67

8.96

BD Biosciences

610636

mouse monoclonal

1:500

Syntaxin7

55.981

21.623 (ROV)

78.6

4.45

Jahn Department

 

mouse monoclonal 109.1

1:100

Syntaxin8

13.045

5.342 (ROV)

~100–200**

 

Synaptic Systems

110 083

rabbit polyclonal

1:100

TfR

21.293

0.719 (SEM)

***

 

Abcam

ab84036

rabbit polyclonal

1:100

TOM20

13.826

0.379 (SEM)

528.81

155.68

Sigma-Aldrich

WH0009804M1

mouse monoclonal

1:200

Tubulin

21.351

1.367 (SEM)

12056

615.3

Synaptic Systems

302 203

rabbit polyclonal

1:3000

VAMP1

17.671

1.315 (ROV)

3884.3

181.95

Synaptic Systems

104 002

rabbit polyclonal

1:500

VAMP2

21.158

1.199 (SEM)

26448

661.62

Synaptic Systems

104 211

mouse monoclonal

1:500

VAMP2

29.226

1.068 (SEM)

26448

661.62

Synaptic Systems

104 211

mouse monoclonal

1:1000

VAMP4

15.872

0.792 (SEM)

100.59

10.03

Synaptic Systems

136 002

rabbit polyclonal

1:100

VAMP7

8.528

0.924 (SEM)

~100–200**

 

Abcam

ab68776

rabbit polyclonal

1:100

vATPase

129.622

10.131 (SEM)

742.37

32.97

Synaptic Systems

109 002

rabbit polyclonal

1:100

VDAC1

19.532

1.269 (SEM)

14422.99

720.71

Santa Cruz

sc32063

rabbit polyclonal

1:100

Vglut1/2

13.518

1.565 (SEM)

8254.1

224.3

Synaptic Systems

135 503

rabbit polyclonal

1:100

Vti1a

12.390

0.487 (SEM)

50.55

2.51

BD Biosciences

611220

mouse monoclonal

1:100

Vti1a

17.383

5.03 (SEM)

50.55

2.51

BD Biosciences

611220

mouse monoclonal

1:100

  1. Average numbers of antibodies per synaptosome and respective information about the conditions of immunostainings from which the numbers have been derived. The values are mean values obtained either from typically several hundreds of analyzed synapses or several experiments, which were done by multiple investigators. Column C shows either the standard error of the mean (SEM; in case of averaged synapses) or the range of values (ROV; in case of averaged experiments).
  2. *Based on Wilhelm et al.9.
  3. **Numbers estimated by the authors based on the copy numbers of functionally-related SNAREs, taking into account the high correlation between SNARE numbers in different pathways, as observed in the original work cited here.
  4. ***Not determined in the original work (Wilhelm et al.9), but currently under measurement in the Rizzoli laboratory for future references.
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