Table 2 Numbers of primary antibodies per synaptosome for over 100 targets (continued).

From: Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins

Fixation

Blocking

Permeabilization

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.3% Tween 20

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

4% PFA

2.5% BSA

0.1% Triton X-100

3% Glyoxal

2.5% BSA

0.3% Tween 20

4% PFA

2.5% BSA

0.1% Triton X-100

  1. Additional information about the conditions of immunostainings.
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