Table 3 Antibodies used for the immunostaining of cultured neurons and synaptosomes.
Target protein | Species | Company | Dilution |
|---|---|---|---|
primary antibodies | |||
Synaptophysin | guinea pig | SySy (#101004) | 1:300 |
Bassoon | mouse | Enzo (ADI-VAM-PS003-F) | 1:100 |
SNAP25 | rabbit | SySy (#111002) | 1:500 |
Synapsin | rabbit | SySy (#106002) | 1:500 |
Synaptogyrin | rabbit | SySy (#103002) | 1:200 |
Syntaxin1 | rabbit | SySy (#110302) | 1:100 |
Syntaxin12/13 | rabbit | SySy (#110133) | 1:200 |
Synaptotagmin1 | rabbit | SySy (#105102) | 1:100 |
VAMP2 | rabbit | SySy (#104202) | 1:500 |
vGlut1/2 | rabbit | SySy (#135503) | 1:100 |
secondary antibodies | |||
anti-guinea pig IgG (Alexa488) | donkey | Dianova (#706-545-148] | 1:100 |
anti-mouse IgG (Cy5) | donkey | Dianova (#715-175-150) | 1:100 |
anti-rabbit IgG (Cy3) | donkey | Dianova (#711-165-152) | 1:100 |
