Figure 6 | Scientific Reports

Figure 6

From: Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site

Figure 6

Gene expression analysis of C. reinhardtii IL, A250R and S264K strains. (A) Schematic representation of carotenoids (blue arrows) and PQ (red arrow) biosynthetic pathways (adapted from38,39). In italics are the enzymes analysed by qRT–PCR of samples under HL/HT treatment. (B) Gene expression analyses of carotenoids and PQ/tochopherol biosynthetic pathway genes in response to HL/HT treatment. Time course of mRNA expression levels of phytoene synthase (psy), phytoene desaturase (pds), lycopene β-cyclase (lycβ), lycopene ε-cyclase (lycε), carotene β-hydroxylase (chyβ), violaxanthin de-epoxidase (vdr), zeaxanthin epoxidase (zep), homogentisate solanesyl transferase (hst1) and methylphytyl benzoquinone methyltransferase (mpqb-mt) by qRT–PCR. rack1 gene was used as the endogenous control. The results are an average of three biological replicates, ± SE, n = 9.

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