Figure 6
From: PAR-1 is a novel mechano-sensor transducing laminar flow-mediated endothelial signaling
The effects of PAR-1 siRNA on the laminar flow-induced phosphorylations of Akt, AMPK, ERK5, HDAC5, and eNOS in HUVECs. Cells transfected with control or PAR-1 siRNA (100 pmol/L) for 2 days were exposed to laminar flow for 30 or 90 minutes. (A, B) Protein levels of pAKT, AKT, pAMPK, AMPK, pERK5, ERK5, pHDAC5, HDAC5, PAR-1, and tubulin were assayed by Western blotting. (C, D) Protein levels of peNOS and tubulin were determined by immunoblotting with specific antibodies, respectively. The results shown are representative of three independent experiments. *P < 0.05; **P < 0.01 vs. control siRNA transfected HUVECs. #P < 0.05 vs. control siRNA transfected HUVECs exposed to laminar flow (12 dynes/cm2). Cells were used for experiments at passage 4, 5 and 6.
